Author's response to reviews Title: Tannin extracts from immature fruits of Terminalia chebula Fructus Retz. promote cutaneous wound healing in rats Authors:

The abstract is poorly written and complete abstract to be rewritten Background: Tannins extracted from immature fruits of Terminalia chebula Fructus Retz. are considered to be effective components to promote wound healing. This study aims to study optimal extraction and purification technology (OEPT) of tannins and study the use of the drug for the treatment of a cutaneuous wound of rat as well as its antibacterial effects. Methods: The content of tannin extracts was measured by the casein method and antibacterial ability was studied by the microdilution method in vitro. In wound healing experiment, animals in group I、II and III were treated with vaseline ointment、tannin extracts(tannin content:81%) and erythromycin ointment 5mg per wound, respectively. Selected pharmacological and biochemical parameters were used to evaluated wound healing. Results: Content of tannin extracts after optimal extraction and purification was increased to 81%. Tannin extracts showed inhibition of Staphylococcus aureus and Klebsiella Pneumonia in vitro. On day 7 and 10 after excision wounds creation, the percent of wound contraction of group II was higher than that of groupI. On day 3, 7, and 10 after wounds creation, the wound healing quality of groupIIwas better than that of groupIin terms of granulation formation and collagen organization. On day 3 after wound creation, the vascular endothelial growth factor expression of groupIIwas higher than that of groupI. Conclusion: The results suggest that tannin extracts from dried immature fruits of. can promote cutaneous wound healing in rats, which probably results from a powerful antibacterial and angiogenic activity of the extracts. 3 Introduction Authors should clearly state the reason for selecting tannins from Terminaliachebula Fructus Retz to show the wound healing activity It has been accredited for a long history of use in Ayurvedic and also Thai folk medicines as a natural remedy for skin diseases, wound and rejuvenation[7] [7] M. Chulasiri,P. Wanaswas, D. Sriaum, S. Nakamat, Y. Wongkrajang, B. Kongsaktrakoon, S. Phornchirasilp, S. Songchitsomboon, D. Leelarungrayub. Utilizing hydroglycolic extract from myrobalan fruits to counteract reactive oxygen species. Int J Cosmetic Sci 2011; 33(4):371-376. 4Why authors selected only two microorganisms to study the antimicrobial activity? The research group has been dedicated to the research on natural drugs for the treatment of infected wound, the most typical clinical wound infection is caused by Staphylococcus aureus, however, Gram-negative Klebsiella has emerged frequently in China’s clinical infected wound reports over recent years, and the Klebsiella is drug-resistant and is also the strain detected mainly in superbacteria. Furthermore, the research group is quite familiar with the culture of the two bacteria and the action mechanism of antibacterial drugs on them, so these two bacteria are selected.